Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen H2 Histamine Receptor Membrane Preparation, 1 vial. (HTS086M)
The ChemiScreen H2 Histamine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of HRH2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay CysLT2 Leukotriene Receptor Frozen Cells, 2 Vials of Cells. (HTS088RTA)
The Ready-to-Assay CysLT2 Leukotriene Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. leukotriene) based activation of CYSLTR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CYSLTR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CYSLTR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay FP Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS093RTA)
The Ready-to-Assay FP Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin F2a) based activation of PTGFR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGFR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGFR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen S1P3 Lysophospholipid Receptor Stable Cell Line, 2 vials. (HTS097C)
The ChemiScreen S1P3 Lysophospholipid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. S1P) based activation of S1PR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses S1PR3 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR3, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen β1 Adrenoceptor Receptor Stable Cell Line, 2 vials. (HTS104C)
The ChemiScreen β1 Adrenoceptor Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRB1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRB1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of ADRB1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay 5-HT2B Serotonin Receptor Frozen Cells, 2 Vials of Cells. (HTS109RTA)
The Ready-to-Assay 5-HT2B Serotonin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. serotonin/5-HT) based activation of HTR2B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR2B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HTR2B, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay M2 Acetylcholine (Muscarinic) Receptor Frozen Cells, 2 Vials of Cells. (HTS115RTA)
The Ready-to-Assay M2 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen M4 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS117M)
The ChemiScreen M4 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM4 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen LPA1 Lysophopholipid Receptor Stable Cell Line, 2 vials. (HTS089C)
The ChemiScreen LPA1 Lysophopholipid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. LPA) based activation of LPAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LPAR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of LPAR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen S1P3 Lysophospholipid Receptor Membrane Preparation, 1 vial. (HTS097M)
The ChemiScreen S1P3 Lysophospholipid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of S1PR3 surface expression. ChemiScreen membrane preps can be used to screen for GPCR activity using GTPγS assays. Increased GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen δ Opioid Receptor Membrane Preparation, 1 vial. (HTS100M)
The ChemiScreen δ Opioid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of OPRD1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay 5-HT6 Serotonin Receptor Frozen Cells, 2 Vials of Cells. (HTS111RTA)
The Ready-to-Assay 5-HT6 Serotonin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. serotonin/5-HT) based activation of HTR6 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR6 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HTR6, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay BB1 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS123RTA)
The Ready-to-Assay BB1 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells, 2 Vials of Cells. (HTS135RTA)
The Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. free fatty acids) based activation of FFAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR3 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen EP4 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS142C)
The ChemiScreen EP4 Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.